36-2698-20 36-2698
Anti-Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator) Monoclonal Antibody(Clone: LAG3/3261)
Abeomics
DESCRIPTION
LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.
DETAILS
- Gene: LAG3
- App Id: ELISA
- Gene Id: 3902
- Isotype: Mouse IgG2a, kappa
- Clone Name: LAG3/3261
- Uniprot Id: P18627
- Host Animal: Mouse
- Clonality Id: Monoclonal
- Reactivity Id: Human
- Product Content: 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
- Alternative Name: CD223; FDC protein; LAG-3
- Application Note: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA);
- Storage Condition: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
- Immunogen Information: Recombinant full-length human LAG3 protein