32-13138-2 32-13138
CDH5 Human
Abeomics
Image
DESCRIPTION
Source: Sf9, Insect cells.Sterile filtered colorless solution.Cadherin-5 (CDH5) belongs to the atypical/type 2 subgroup of Cadherin homophilic adhesion proteins. Cadherin-5 is a calcium-dependent cell-cell adhesion molecule, which is comprised of 5 extracellular cadherin repeats, a transmembrane region & a highly conserved cytoplasmic tail. Cadherin-5 protein plays a role in the formation, maturation & remodeling of the vascular wall. CDH5 is generally considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. Moreover, the CDH5 protein regulates or is regulated by VEGF R2, type 1 & type 2 TGF-beta receptors, & other endothelial junction proteins such as JAM-C, Claudin-5, & N-Cadherin. CDH5 also connects with alpha-catenin forming a link to the cytoskeleton. CDH5 functions jointly with KRIT1 to create & maintain correct endothelial cell polarity & vascular lumen. Furthermore, Cadherin-5 is essential for activation of PRKCZ & for the localization of phosphorylated PRKCZ, PARD3, TIAM1 & RAP1B to the cell junction. The CDH5 gene is located in a gene cluster in a region on the long arm of chromosome 16 which is involved in loss of heterozygosity events in breast & prostate cancer.Cadherin 5 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 810 amino acids (29-599 a.a.) & having a molecular mass of 91.5kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). CDH5 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus & purified by proprietary chromatographic techniques.
DETAILS
- Amino Acid: NPAQRDTHSL LPTHRRQKRD WIWNQMHIDE EKNTSLPHHV GKIKSSVSRK NAKYLLKGEY VGKVFRVDAE TGDVFAIERL DRENISEYHL TAVIVDKDTG ENLETPSSFT IKVHDVNDNW PVFTHRLFNA SVPESSAVGT SVISVTAVDA DDPTVGDHAS VMYQILKGKE YFAIDNSGRI ITITKSLDRE KQARYEIVVE ARDAQGLRGD SGTATVLVTL QDINDNFPFF TQTKYTFVVP EDTRVGTSVG SLFVEDPDEP QNRMTKYSIL RGDYQDAFTI ETNPAHNEGI IKPMKPLDYE YIQQYSFIVE ATDPTIDLRY MSPPAGNRAQ VIINITDVDE PPIFQQPFYH FQLKENQKKP LIGTVLAMDP DAARHSIGYS IRRTSDKGQF FRVTKKGDIY NEKELDREVY PWYNLTVEAK ELDSTGTPTG KESIVQVHIE VLDENDNAPE FAKPYQPKVC ENAVHGQLVL QISAIDKDIT PRNVKFKFIL NTENNFTLTD NHDNTANITV KYGQFDREHT KVHFLPVVIS DNGMPSRTGT STLTVAVCKC NEQGEFTFCE DMAAQVGVSI QLEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH.
- Purification: Greater than 90.0% as determined by SDS-PAGE.
- Product Content: CDH5 solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) & 10% glycerol.
- Alternative Name: Cadherin 5, VE-Cadherin, Cadherin 5 Type 2 VE-Cadherin (Vascular Epithelium), CDH5, Cadherin 5 Type 2 (Vascular Endothelium), Vascular Endothelial Cadherin, 7B4 Antigen, Endothelial-Specific Cadherin, Cd144 Antigen, CD144 Antigen, Cadherin-5, CD144, 7B4.
- Storage Condition: Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.