10692-H20B1
Human c-MET / HGFR Protein (aa 956-1390, His & GST Tag)
Sino Biological
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DESCRIPTION
A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
DETAILS
- Tag: N-GST & His
- Purity: > 90 % as determined by SDS-PAGE
- Source: Baculovirus-Insect Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: 1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized human HGFR (aa 956-1390) (Cat: 10692-H20B1) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his (Cat: 10463-H08H) with a linear range of 15.6-125 ng/ml.
- Molecule: MET
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT
- Molecular Mass: The recombinant human MET /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
- Accession Number: P08581-1
- Predicted N Terminal: Met