DESCRIPTION

• Mw: 96.7 kDa
• Tag: Myc-DDK
• Accn: NM_001270941
• Type: Human Tagged ORF Clone
• Refseq: NM_001270941.2
• Status: 3 Weeks*
• Symbol: JAKMIP2
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 9832
• Orf Size: 2460 bp
• Synonyms: JAMIP2; NECC1
• Refseq Orf: 2463 bp
• Uniprot Id: Q96AA8
• Refseq Size: 9272 bp
• Availability: 3 Weeks
• Cytogenetics: 5q32
• Gene Summary: The protein encoded by this gene is reported to be a component of the Golgi matrix. It may act as a golgin protein by negatively regulating transit of secretory cargo and by acting as a structural scaffold of the Golgi. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC234931 representing NM_001270941 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGTCCAAGAAAGGGCGAAATAAGGGCGAGAAGCCCGAGGCACTCATTGTTGCCCTTCAAGCTGCCAATG AAGACCTCAGGACCAAGCTCACAGACATTCAGATAGAGCTGCATCAAGAGAAGTCCAAGGTATCAAAGCT TGAAAGAGAGAAGACTCAAGAAGCGAAGAGGATTCGTGAGCTGGAGCAGCGCAAGCACACGGTGCTGGTG ACAGAACTCAAAGCCAAGCTCCATGAGGAGAAGATGAAGGAGCTGCAGGCTGTGAGGGAGAACCTTATCA AGCAGCACGAGCAGGAAATGTCAAGGACGGTGAAGGTACGTGATGGAGAGATCCAGAGGCTCAAGTCTGC TCTCTGTGCTCTCCGCGACGGCAGCAGTGACAAAGTAAGGACAGCGCTCACCATTGAGGCCCGGGAGGAG GCCCGGAAACTGTTTGACACAGAGCGCCTTAAGCTCTTACAGGAAATTGCGGACCTGAAAACGGCCAAGA AGCAGGTGGACGAGGCTCTGAGCAATATGATCCAAGCAGATAAAATCAAGGCTGGGGACCTTCGGAGTGA GCATCAGTCCCACCAAGAAGCCATCTCGAAGATCAAGTGGGAGTCGGAGCGGGATATTCGGAGGCTGATG GATGAAATCAAAGCCAAGGACAGGATCATCTTTTCCCTGGAAAAGGAACTGGAGACCCAGACAGGCTATG TACAGAAACTCCAACTTCAGAAGGAGGCTTTGGACGAACAACTCTTTCTGGTCAAGGAGGCTGAGTGCAA CATGAGCAGCCCAAAACGAGAAATTCCAGGAAGGGCAGGTGATGGTTCCGAACACTGCAGCAGTCCTGAT TTGCGAAGAAATCAAAAGAGAATAGCTGAATTGAATGCCACTATAAGAAAATTAGAAGACAGGAATACCT TGCTTGGAGATGAACGAAATGAACTGTTAAAACGTGTGCGGGAAACCGAAAAGCAATGTAAACCTCTCCT GGAAAGGAACAAGTGCCTCGCCAAGAGAAACGATGAACTGATGGTGTCCTTGCAGCGCATGGAAGAAAAA CTAAAAGCCGTTACCAAGGAAAATTCAGAAATGAGAGAAAAAATAACATCCCATCCACCCCTGAAGAAAT TAAAATCTCTGAATGACCTCGACCAAGCTAATGAAGAACAAGAAACAGAGTTTCTAAAACTTCAGGTCAT TGAGCAACAGAACATTATTGATGAGCTCACAAGGGACCGAGAAAAGCTCATCCGTAGAAGAAAGCATAGA AGAAGTTCCAAGCCAATTAAGAGGCCTGTTTTGGACCCGTTTATTGGCTATGATGAGGACTCTATGGATT CAGAGACATCATCCATGGCCTCATTTAGAACAGACAGAACACCAGCTACTCCTGATGATGACTTGGATGA AAGTTTAGCAGCTGAAGAATCTGAACTAAGATTTCGACAATTAACAAAAGAATATCAGGCCCTCCAAAGA GCATATGCCCTCCTACAGGAGCAGACGGGAGGCATCATCGACGCTGAACGAGAAGCCAAGGCTCAAGAAC AGCTCCAAGCAGAGGTGCTAAGGTATAAAGCCAAAATTGAAGACCTGGAAGCGACTCTGGCTCAGAAAGG GCAGGATTCACACTGGGTAGAAGATAAACAACTTTTCATTAAGAGAAACCAGGAGCTTTTAGAAAAGATA GAAAAACAGGAGGCAGAAAATCACCGGTTACAACAAGAACTACAGGACGCCAGAGACCAGAATGAGCTGC TGGAGTTTCGAAACCTAGAGCTAGAAGAGAGAGAGAGACGATCCCCTCCATTTAATCTCCAAATTCACCC ATTCTCAGATGGTGTGAGTGCTCTACAGATCTACTGTATGAAAGAAGGTGTTAAGGATGTGAACATCCCT GATCTCATAAAGCAGCTTGATATCTTGGGTGATAATGGGAATTTAAGAAATGAAGAACAAGTGGCCATAA TTCAGGCCAGCACTGTGCTGTCCCTGGCAGAGAAGTGGATCCAGCAGATTGAAGGAGCTGAGGCTGCCCT ACACCAGAAAATGATGGAATTGGAAAGTGACATGGAACAGTTCTGCAAAATAAAAGGCTATCTGGAGGAA GAACTAGACTACAGAAAACAAGCTCTTGACCAAGCATATATGAGAATCCAGGAACTAGAAGCTACTTTGT ACAATGCTCTACAGCAAGAAACTGTTATCAAGTTTGGTGAATTATTAAGTGAAAAACAGCAAGAGGAGCT GAGGACGGCAGTAGAAAAGTTACGGCGGCAAATGCTGAGGAAGAGCAGAGAGTATGACTGTCAGATTCTT CAGGAGAGAATGGAGCTCTTACAGCAAGCCCATCAGAGAATTCGTGACTTAGAAGATAAAACAGACATCC AGAAAAGACAAATAAAAGACTTAGAAGAAAAGTTTCTGTTTCTATTCTTGTTCTTCTCTCTTGCCTTTAT TCTATGGCCT ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC234931 representing NM_001270941 Red =Cloning site Green =Tags(s) MSKKGRNKGEKPEALIVALQAANEDLRTKLTDIQIELHQEKSKVSKLEREKTQEAKRIRELEQRKHTVLV TELKAKLHEEKMKELQAVRENLIKQHEQEMSRTVKVRDGEIQRLKSALCALRDGSSDKVRTALTIEAREE ARKLFDTERLKLLQEIADLKTAKKQVDEALSNMIQADKIKAGDLRSEHQSHQEAISKIKWESERDIRRLM DEIKAKDRIIFSLEKELETQTGYVQKLQLQKEALDEQLFLVKEAECNMSSPKREIPGRAGDGSEHCSSPD LRRNQKRIAELNATIRKLEDRNTLLGDERNELLKRVRETEKQCKPLLERNKCLAKRNDELMVSLQRMEEK LKAVTKENSEMREKITSHPPLKKLKSLNDLDQANEEQETEFLKLQVIEQQNIIDELTRDREKLIRRRKHR RSSKPIKRPVLDPFIGYDEDSMDSETSSMASFRTDRTPATPDDDLDESLAAEESELRFRQLTKEYQALQR AYALLQEQTGGIIDAEREAKAQEQLQAEVLRYKAKIEDLEATLAQKGQDSHWVEDKQLFIKRNQELLEKI EKQEAENHRLQQELQDARDQNELLEFRNLELEERERRSPPFNLQIHPFSDGVSALQIYCMKEGVKDVNIP DLIKQLDILGDNGNLRNEEQVAIIQASTVLSLAEKWIQQIEGAEAALHQKMMELESDMEQFCKIKGYLEE ELDYRKQALDQAYMRIQELEATLYNALQQETVIKFGELLSEKQQEELRTAVEKLRRQMLRKSREYDCQIL QERMELLQQAHQRIRDLEDKTDIQKRQIKDLEEKFLFLFLFFSLAFILWP TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin