DESCRIPTION

• Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles.
• Category: PARP/Assay Kit
• Description: The PARP12 Chemiluminescent Assay Kit is designed to measure PARP12 activity for screening and profiling applications. PARP12 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP12 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP12 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP12 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
• Methodology: Chemiluminescent
• Supplied As: The PARP12 assay kit comes in a convenient 96-well format, with purified PARP12 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and high throughput applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 80568, 80567, 80561, 80560
• Contraindications: The PARP12 Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.
• Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
• Scientific Category: PARP