Monoclonal Antibody to Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker)

DESCRIPTION

Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

DETAILS

Gene: MYH11
App Id: FACS,IF,IHC
Antigen: Smooth Muscle Myosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker)
Gene Id: 4629
Isotype: Mouse IgG1, kappa + Mouse IgG1, kappa
Format Id: Purified
Clone Name: MYH11/923 + SMMS-1
Uniprot Id: P35749
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human, Rat
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: MYH11||KIAA0866
Application Note: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Recombinant human MYH11 protein (MYH11/923); Human uterus extract (SMMS-1)