DESCRIPTION

• Form: Protein G Purified
• Gene: NEFM
• Host: Mouse
• Rrid: AB_2492163
• Type: Primary Antibody
• Clone: 3H11
• Buffer: PBS + 10 mM NaN3.
• Target: Neurofilament NF-M
• Isotype: IgG1
• Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
• Shipping: Blue Ice
• Synonyms: 150 kDa Neurofilament Medium antibody, 160 kDa neurofilament protein antibody, Medium polypeptide 150kDa antibody, NEF3 antibody, Neurofilament 3 antibody, Neurofilament 3 medium antibody, Neurofilament medium polypeptide antibody, Neurofilament protein M antibody, Neurofilament triplet M protein antibody, Neurofilament-3 (150 kD medium) antibody, NF-M antibody, NFM antibody
• Clonality: Monoclonal
• Immunogen: Preparation containing the extreme C-terminus of rat NF-M, expressed in and purified from E. Coli.
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~145 kDa neurofilament M protein.
• Applications: WB, IHC, ICC
• Ncbi Gene Id: 24588
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 145
• Production Notes: Protein G purified tissue culture supernatant.
• Dilution Range Wb: 1:1000
• Immunogen Species: Rat
• Dilution Range Icc: 1:100-1:500
• Dilution Range Ihc: 1:500-1:5000
• Species Reactivity: Bovine, Chicken, Human, Mouse, and Rat
• Target Description: Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: P12839

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