DESCRIPTION

• Nature: Recombinant
• Purity: >95%
• Target: Tau-441 (2N4R) Wild-Type
• Category: Protein
• Conjugate: No Tag
• References: 1. Goedert et al. 1989. Multiple Isoforms of Human Microtubule-associated Protein Tau: Sequences and Localization in Neurofibrilary Tangles of Alzheimer’s Disease. Neuron. doi: 10.1016/0896-6273(89)90210-9. 2. Iqbal K., Liu F., and Gong C.X. 2016. Tau and neurodegenerative disease: The story so far. Nat. Rev. Neurol. DOI: 10.1038/nrneurol.2015.225 3. Tepper et al. 2014. Oligomer Formation of Tau Protein Hyperphophorylated in Cells. The Journal of Biological Chemistry, DOI 10.1074/jbc.M114.611368
• Applications: WB | SDS PAGE | In vitro Assay
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
• Protein Size: 45.84 kDa
• Purification: Ion-exchange Purified
• Concentration: 2 mg/ml
• Protein Length: Full Length (1-441 aa)
• Research Areas: Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau
• Storage Buffer: 1X PB pH7.4
• Alternative Names: MAPT, intracellular neurofibrillary tangles, NFTs, paired helical filaments, PHFs, 2N4R
• Cite This Product: Human Recombinant Tau-441 (2N4R) Wild-Type Monomers (Baculovirus/Sf9) (StressMarq Biosciences Inc. | Victoria BC CANADA | Catalog # SPR-496B)
• Expression System: Baculovirus (Sf9)
• Species Full Name: Human
• Amino Acid Sequence: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
• Storage Temperature: -80ºC
• Shipping Temperature: Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
• Scientific Background: Brain-specific tau isoforms vary in the number of N-terminal inserts and C- terminal repeat domains due to alternative splicing of exons; the 2N4R isoform of tau is expressed in adult brain yet is absent from the fetal brain (1). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as Alzheimer’s Disease (AD) are characterized by neurofibrillary tangles containing hyper-phosphorylated tau fibrils (2). Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and Western Blots with phospho-specific antibodies(3). Sf9/Baculovirus-expressed Tau 2N4R will readily form oligomers and fibrils in the absence of heparin.
• Certificate of Analysis: Protein certified >95% pure on SDS-PAGE & Nanodrop analysis. Low endotoxin <5 EU/mL @ 2mg/mL.