DESCRIPTION

• Mw: 35.3 kDa
• Tag: Myc-DDK
• Accn: NM_001272088
• Type: Human Tagged ORF Clone
• Refseq: NM_001272088.2
• Status: 3 Weeks*
• Symbol: EAAT4
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 6511
• Orf Size: 936 bp
• Synonyms: EAAT4
• Refseq Orf: 939 bp
• Uniprot Id: P48664
• Refseq Size: 1867 bp
• Availability: 3 Weeks
• Cytogenetics: 19p13.12
• Gene Summary: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7791878). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).[UniProtKB/Swiss-Prot Function]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC233725 representing NM_001272088 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGAGCAGCCATGGCAACAGCCTGTTCCTGCGGGAGAGCGGCCAGCGGCTGGGCCGGGTGGGCTGGCTGC AGCGGCTGCAGGAAAGCCTGCAGCAGAGAGCACTGCGCACGCGCCTGCGCCTGCAGACCATGACCCTCGA GCACGTGCTGCGCTTCCTGCGCCGAAACGCCTTCATTCTGCTGACGGTCAGCGCCGTGGTCATTGGGGTC AGCCTGGCCTTTGCCCTGCGCCCATATCAGCTCACCTACCGCCAGATCAAGTACTTCTCTTTTCCTGGAG AGCTTCTGATGAGGATGCTGCAGATGCTGGTGTTACCTCTCATTGTCTCCAGCCTGGTCACAGGTATGGC ATCCCTGGACAACAAGGCCACGGGGCGGATGGGGATGCGGGCAGCTGTGTACTACATGGTGACCACCATC ATCGCGGTCTTCATCGGCATCCTCATGGTCACCATCATCCATCCCGGGAAGGGCTCCAAGGAGGGGCTGC ACCGGGAGGGCCGGATCGAGACCATCCCCACAGCTGATGCCTTCATGGACCTGATCAGAAATATGTTTCC ACCAAACCTTGTGGAGGCCTGCTTCAAACAGTTCAAGACGCAGTACAGCACGAGGGTGGTAACCAGGACC ATGGTGAGGACAGAGAACGGGTCTGAGCCGGGTGCCTCCATGCCTCCTCCATTCTCAGTGGAGAACGGAA CCAGCTTCCTGGAAAATGTCACTCGGGCCTTGGGTACCCTGCAGGAGATGCTGAGCTTTGAGGAGACTGT ACCCGTGCCTGGCTCCGCCAATGGCATCAACGCCCTGGGCCTCGTGGTCTTCTCTGTGGCCTTTGGGCTG GTCATTGGTGGCATGAAACACAAGGGCAGAGTCCTCAGGGACTTCTTCGACAGCCTCAATGAGGCTATTA TGAGGCTGGTGGGCATCATTATCTGG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC233725 representing NM_001272088 Red =Cloning site Green =Tags(s) MSSHGNSLFLRESGQRLGRVGWLQRLQESLQQRALRTRLRLQTMTLEHVLRFLRRNAFILLTVSAVVIGV SLAFALRPYQLTYRQIKYFSFPGELLMRMLQMLVLPLIVSSLVTGMASLDNKATGRMGMRAAVYYMVTTI IAVFIGILMVTIIHPGKGSKEGLHREGRIETIPTADAFMDLIRNMFPPNLVEACFKQFKTQYSTRVVTRT MVRTENGSEPGASMPPPFSVENGTSFLENVTRALGTLQEMLSFEETVPVPGSANGINALGLVVFSVAFGL VIGGMKHKGRVLRDFFDSLNEAIMRLVGIIIW TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Transmembrane
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin