32-1517
mIL 33 Recombinant Protein
Abeomics
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DESCRIPTION
Source : Escherichia Coli.
Interleukin33 Mouse recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids & having a molecular mass of 17.5 kDa.
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines & acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B & MAP kinases & induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 & leads to severe pathological changes in mucosal organs & in vitro, it can be divided to N-terminal fragment of 12kDa & C-terminal fragment of 18kDa by cleavage of caspase-1.
DETAILS
- Amino Acid: SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLH&K DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI.
- Purification: Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
- Product Content: The Mouse IL-33 was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH-7.4, 150mM sodium chloride, 1mM EDTA & 2mM b-ME.
- Alternative Name: Interleukin 33||DVS27||NF-HEV||NKHEV||C9orf26||Interleukin-1 family member 11||IL- 1F11||Nuclear factor from high endothelial venules||Il-33||Il1f11||9230117N10Rik||Il33.
- Application Note: It is recommended to reconstitute the lyophilized IL-33 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is < 0.5ng/ml, corresponding to a specific activity of 2,000,000 units/mg.
- Storage Condition: Lyophilized IL-33 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-33 should be stored at 4°C between 2-7 days & for future use below -18°C.Please prevent freeze-thaw cycles.