Anti-CD59 (glycoprotein) Antibody

DETAILS

Form: Protein G Purified
Gene: CD59
Host: Mouse
Rrid: AB_3068376
Type: Primary Antibody
Clone: M011
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: CD59 (glycoprotein)
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: CD59 glycoprotein, 1F5 antigen, HRF-20, HRF20, MAP-IP, MAC inhibitory protein, MEM43, MACIF, MIRL, MIC11, MIN1, MIN2, MIN3, MSK21
Clonality: Mouse Monoclonal
Immunogen: Clone M011 was generated from a proprietary antigen related to the mature form of human CD59 from the MDA-MB-231 breast carcinoma cell line.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: Clone M011 detects 15-20 kDa* bands corresponding to CD59 on SDS-PAGE immunoblots of native human MDA-MB-231, A549, A431, and MCF7 cell lysates, as well as recombinant human CD59 protein. The antibody does not detect denatured CD59. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemical labeling and immunoprecipitation. In addition, the antibody labels live, unfixed A549 and MeWo cells.
Applications: WB, ICC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 15-20
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human
Target Description: CD59 is a GPI-anchored membrane protein that is an inhibitor of the complement membrane attack complex (MAC). CD59 binds to complement components C8 and C9, preventing C9 polymerization and insertion into membranes. Rare cases of CD59 deficiency have been reported to cause paroxysmal nocturnal hemoglobinuria in human patients. Expression of CD59 on tumor cells and viral infected cells makes them resist antibody-dependent complement-mediated lysis. Inhibitors of CD59 expression or activity may suppress tumor cell resistance to complement-mediated attack, and these technologies have been actively pursued for therapeutic applications. In addition, CD59 may regulate insulin secretion by modulating exocytosis, and a glycated form of CD59 with no MAC inhibitory activity is found in diabetic patients.
Uniprot Number Immunogen Species: P13987
Post Translational Modification Type: GOmAb