Anti-Villin (GI-Mucosal & Urogenital Brush Border Marker) Recombinant Mouse Monoclonal Antibody (Clone:rVIL1/1325)

DESCRIPTION

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

DETAILS

Gene: VIL1
App Id: IHC
Gene Id: 7429
Isotype: Mouse IgG1, kappa
Format Id: Purified
Clone Name: rVIL1/1325
Uniprot Id: P09327
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Protein A/G
Reactivity Id: Human
Product Content: 200µg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Alternative Name: VIL1; Villin-1; Villin1
Application Note: Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Immunogen Information: Recombinant fragment of human Villin (aa 179-311) (exact sequence is proprietary)