DESCRIPTION

• Format: Aqueous buffer solution
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles.
• Category: Deacetylase/Assay Kit
• References: 1. K. Ito et al. Proc. Natl. Acad. Sci. USA 2002 99: 8921 2. B. Heltweg and M. Jung Anal. Biochem. 2002 302: 175 3. S. Milutinovic et al. J. Biol. Chem. 2002 277: 20974
• Description: The Kinetic HDAC assay developer is an essential reagent for signal generation in Kinetic HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates. The developer sensitizes the deacetylated substrate so that subsequent treatment with the HDAC assay developer produces a fluorophore.
• Supplied As: A 10x concentrate (1 ml) with 10 ml dilution buffer.
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Study of HDAC kinetics, regulation and inhibitor sensitivity. Suitable for use with the Kinetic HDAC assay kits: • Kinetic HDAC1 Assay Kit • Kinetic HDAC2 Assay Kit • Kinetic HDAC3 Assay Kit
• Product Type: Assay Kit Component
• Biosafety Level: Not applicable (BSL-1)
• Storage Stability: Store in aliquots, only as the 10x concentrate, at -70°C. Concentrate is stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing).
• Scientific Category: Deacetylase
• Instructions for Use: Dilute 10x developer to 1x with dilution buffer before use. Add 50 µl 1x developer per 50 µl HDAC reaction (1:1, total volume 100 µl).