• Form: Antigen Affinity Purified from Pooled Serum
• Gene: GAP43
• Host: Rabbit
• Rrid: AB_2492123
• Type: Primary Antibody
• Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Target: Gap-43 (Ser41)
• Isotype: IgG
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Shipping: Blue Ice
• Synonyms: Axonal membrane protein GAP 43 antibody, Axonal membrane protein GAP-43 antibody, B 50 antibody, Calmodulin binding protein P 57 antibody, F1 antibody, GAP 43 antibody, GAP-43 antibody, GAP43 antibody, Growth Associated Protein 43 antibody, Growth-associated protein 43 antibody, Nerve Growth Related Peptide antibody, Nerve growth related peptide GAP43 antibody, NEUM_HUMAN antibody, Neural phosphoprotein B 50 antibody, Neural phosphoprotein B-50 antibody, Neuromodulin antibody, Neuron growth associated protein 43 antibody, PP46 antibody, Protein F1 antibody, QtrA-11580 antibody, QtrA-13071 antibody
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41of rat GAP43, conjugated to keyhole limpet hemocyanin (KLH).
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~50 kDa Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, which may be a Gap-43 aggregate or oligomer. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
• Applications: WB
• Ncbi Gene Id: 29423
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 50
• Production Notes: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Dilution Range Wb: 1:1000
• Immunogen Species: Rat
• Species Reactivity: Mouse and Rat
• Target Description: Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser-41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).
• Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: P07936
• Post Translational Modification Type: Phosphorylated
• Post Translational Modification Residue: Ser41

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