Papain-like Protease (SARS-CoV-2) Assay Kit: Deubiquitinase Activity

DESCRIPTION

The Papain-like Protease Assay Kit: Deubiquitinase Activity is designed to measure Papain-like Protease deubiquitinating activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.

DETAILS

Shiptemp: -80°C (dry ice)
Synonyms: covid, covid-19, covid19, coronavirus, Severe acute respiratory Coronavirus, PLpro kit, PLP kit, Papain like protease assay
Warnings: Avoid freeze/thaw cycles.
Category: Coronavirus/Assay Kit
Background: Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. Papain-Like Protease (PLPro), also known as PLP, play an essential role in polypeptide processing during virus replication. PLPro, in addition to being crucial during replication via processing of the viral polyprotein, is proposed to be a key enzyme in the sustained pathogenesis of SARS-CoV. This includes deubiquitination (the removal of ubiquitin) from host-cell proteins. The last enzymatic activity result in the antagonism of the host antiviral innate immune response. As a result, PLpro is an important potential target for antiviral drugs that may inhibit viral replication and weaken dysregulation of signalling cascades in infected cells that may lead to cell death in surrounding, uninfected cells. PLPro inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the COVID-19 coronavirus infection.
References: Ewelina Weglarz-Tomczak, et al., 2020. https://doi.org/10.1101/2020.05.17.100768.
Description: The Papain-like Protease Assay Kit: Deubiquitinase Activity is designed to measure Papain-like Protease deubiquitinating activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
Methodology: Fluorogenic
Supplied As: The kit comes in a convenient 96-well format, with purified Papain-like Protease, ubiquitinated fluorogenic substrate, and PLPro assay buffer for 100 enzyme reactions. PLPro inhibitor GRL0617 is also included as a positive control.
Unspsc Code: 41105331
Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
Applications: Great for studying enzyme kinetics and HTS applications.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Related Products: 79931, 100735, 79955, 79995
Storage Stability: At least six months from date of receipt when stored as directed.
Scientific Category: Coronavirus
Instructions for Use: See assay protocol for detailed instructions.