DESCRIPTION

• Aa: 2-858(end)
• Mw: 96 kDa
• Tags: N-terminal FLAG-tag
• Format: Aqueous buffer solution
• Genbank: NM_001098536
• Species: Human
• Uniprot: P45974
• Shiptemp: -80°C (dry ice)
• Synonyms: USP 5, Ubiquitin-Specific Protease 5, Isopeptidase T, IsoT, USP-5
• Warnings: Avoid freeze/thaw cycles.
• Category: Ubiquitination/Deubiquitylating Enzymes
• References: 1. Dayal, S., et al. (2009). J. Biol. Chem. 284(8):5030-41.2. Reyes-Turcu, F.E., et al. (2008). J. Biol. Chem. 283(28):19581-92.
• Description: Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (IsoT), GenBank Accession No. NM_001098536, a.a. 2-858(end) with an N-terminal FLAG-tag, MW=96 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT, and 90 ?g/ml FLAG peptide
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP5 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
• Specific Activity: 14.3 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Ubiquitins: Deubiquitinase (DUB)