DESCRIPTION

• Mw: 108.1 kDa
• Tag: Myc-DDK
• Accn: NM_001195144
• Type: Human Tagged ORF Clone
• Refseq: NM_001195144.2
• Status: 5 Weeks*
• Symbol: ANKRD44
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 91526
• Orf Size: 2979 bp
• Synonyms: PP6-ARS-B
• Refseq Orf: 2982 bp
• Uniprot Id: Q8N8A2
• Availability: 5 Weeks
• Cytogenetics: 2q33.1
• Gene Summary: Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates.[UniProtKB/Swiss-Prot Function]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC231453 representing NM_001195144 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCAGTGCTCAAACTCACCGACCAGCCACCATTGGTTCAGGCAATCTTCAGCGGTGATCCAGAGGAGA TCCGGATGCTCATCCATAAAACTGAAGATGTGAATACTCTGGATTCTGAGAAACGAACCCCTCTTCATGT GGCCGCATTTCTGGGAGATGCAGAGATCATTGAACTCCTGATTTTGTCAGGAGCTCGTGTAAATGCCAAG GACAACATGTGGCTGACTCCACTGCACCGGGCTGTTGCTTCCAGAAGTGAAGAAGCAGTACAGGTTTTGA TTAAGCACTCAGCTGATGTCAATGCAAGGGACAAGAACTGGCAGACCCCTCTTCATGTGGCAGCAGCCAA CAAGGCTGTCAAATGTGCAGAAGTGATCATTCCCCTGCTGAGCAGTGTCAATGTCTCCGACCGAGGGGGG CGCACAGCCTTGCACCATGCGGCTCTGAACGGCCACGTGGAGATGGTCAATTTACTCTTGGCCAAAGGGG CAAATATCAATGCATTTGACAAGAAGGACCGGCGTGCTCTGCACTGGGCAGCATACATGGGCCACTTGGA TGTTGTAGCATTGCTCATTAACCATGGCGCAGAAGTGACCTGTAAGGATAAGAAGGGTTATACCCCTCTG CATGCTGCAGCCTCCAATGGACAGATTAATGTTGTCAAGCATCTCCTGAACCTGGGGGTGGAGATTGATG AAATCAATGTCTATGGAAATACAGCGCTTCACATCGCCTGCTACAATGGACAGGATGCTGTGGTTAACGA GTTGATTGACTACGGTGCTAACGTGAACCAGCCAAACAATAATGGGTTCACCCCTTTGCATTTTGCTGCT GCCTCCACTCATGGTGCTTTGTGTCTTGAATTGTTAGTAAACAACGGGGCAGATGTTAACATTCAGAGTA AAGATGGCAAAAGTCCACTGCACATGACAGCTGTCCATGGAAGGTTCACACGGTCACAGACCCTCATTCA GAATGGAGGTGAAATTGACTGTGTGGATAAGGACGGCAACACTCCTCTCCATGTGGCTGCAAGATACGGT CATGAGCTTTTGATTAACACCTTAATAACCAGCGGAGCTGACACAGCCAAGTGTGGAATCCATAGCATGT TCCCTTTACATTTAGCTGCCCTAAATGCTCACTCTGACTGCTGCAGAAAGTTGTTATCATCGGGCTTTGA AATAGACACCCCAGATAAATTTGGAAGAACGTGCCTTCATGCTGCTGCTGCAGGAGGTAATGTGGAATGT ATAAAACTCTTGCAGAGCAGCGGAGCAGATTTCCATAAAAAGGACAAGTGTGGGAGGACCCCTTTGCACT ATGCAGCTGCGAATTGTCATTTCCACTGTATTGAGACATTAGTGACCACAGGGGCCAACGTTAATGAAAC AGATGACTGGGGACGCACAGCTTTGCATTACGCCGCTGCATCAGACATGGATAGAAATAAGACTATCTTA GGAAATGCCCATGATAATTCAGAAGAACTTGAAAGAGCCAGGGAGCTGAAGGAAAAGGAAGCCACACTAT GTCTAGAGTTTCTGCTTCAAAATGATGCAAATCCATCTATCCGGGACAAGGAAGGTTACAATAGCATACA TTATGCTGCCGCCTATGGGCACAGGCAGTGTCTGGAATTGCTTTTGGAAAGAACAAACAGTGGATTTGAA GAATCAGATTCTGGTGCTACTAAGAGTCCACTCCACTTAGCTGCCTACAATGGGCACCATCAAGCCTTGG AAGTCCTTCTGCAGTCGTTGGTGGACCTGGACATCAGGGATGAGAAAGGCCGCACTGCTCTGGATCTGGC TGCCTTTAAAGGACACACAGAATGTGTGGAAGCGCTTATCAATCAGGGCGCATCCATCTTTGTGAAAGAC AATGTAACCAAAAGAACCCCACTTCATGCCTCAGTAATTAATGGTCACACACTGTGTTTACGGCTGTTGC TAGAAATTGCAGACAACCCGGAGGCGGTCGATGTGAAAGATGCCAAAGGACAAACACCACTGATGCTTGC AGTAGCATATGGACATATTGACGCTGTTTCATTGTTACTTGAAAAGGAAGCCAACGTAGACACTGTTGAC ATCCTAGGATGCACAGCTTTACACAGAGGGATTATGACAGGACACGAGGAATGTGTGCAAATGCTGCTGG AACAAGAAGTGTCAATTCTCTGTAAAGATTCCAGAGGGAGGACGCCCTTGCACTATGCAGCTGCTCGTGG CCACGCCACGTGGCTGAGCGAGCTGCTCCAAATGGCTCTTTCTGAGGAGGACTGTTGTTTCAAAGATAAC CAAGGCTACACGCCGCTGCACTGGGCTTGTTACAATGGTAATGAAAACTGTATAGAGGTACTTTTGGAGC AAAAATGTTTTCGCAAATTTATCGGTAATCCCTTTACTCCACTGCACTGTGCAATAATCAATGATCATGG GAATTGTGCATCATTGCTGCTTGGGGCCATAGATTCCAGTATCGTCAGTTGTAGAGATGACAAAGGCAGG ACACCCCTTCATGCGGCAGCATTTGCTGATCATGTGGAGTGCTTGCAGCTTCTTCTGAGACACAGTGCTC CAGTGAACGCAGTAGATAATTCAGGGAAAACAGCACTGATGATGGCTGCTGAGAATGGGCAGGCAGGCGC TGTGGATATTTTGGTGAACAGTGCCCAGGCTGATCTGACTGTAAAGGATAAGGACTTGAATACACCCTTA CATTTGGCTTGTAGTAAAGGTCATGAAAAATGTGCCTTGTTAATACTTGACAAGATACAAGACGAGAGCC TTATTAATGAAAAAAATAATGCACTGCAGACACCCCTCCACGTCGCTGCGCGCAATGGCTTAAAGGTGGT AGTTGAGGAGTTGCTGGCCAAAGGGGCCTGTGTACTTGCTGTAGATGAAAATGCTTCTAGGTCAAATGGA CCCCGTTCCACACCTGGAACCGCTGTACAAAAAGAAGAA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC231453 representing NM_001195144 Red =Cloning site Green =Tags(s) MAVLKLTDQPPLVQAIFSGDPEEIRMLIHKTEDVNTLDSEKRTPLHVAAFLGDAEIIELLILSGARVNAK DNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGG RTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPL HAAASNGQINVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANVNQPNNNGFTPLHFAA ASTHGALCLELLVNNGADVNIQSKDGKSPLHMTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARYG HELLINTLITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDKFGRTCLHAAAAGGNVEC IKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIETLVTTGANVNETDDWGRTALHYAAASDMDRNKTIL GNAHDNSEELERARELKEKEATLCLEFLLQNDANPSIRDKEGYNSIHYAAAYGHRQCLELLLERTNSGFE ESDSGATKSPLHLAAYNGHHQALEVLLQSLVDLDIRDEKGRTALDLAAFKGHTECVEALINQGASIFVKD NVTKRTPLHASVINGHTLCLRLLLEIADNPEAVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDTVD ILGCTALHRGIMTGHEECVQMLLEQEVSILCKDSRGRTPLHYAAARGHATWLSELLQMALSEEDCCFKDN QGYTPLHWACYNGNENCIEVLLEQKCFRKFIGNPFTPLHCAIINDHGNCASLLLGAIDSSIVSCRDDKGR TPLHAAAFADHVECLQLLLRHSAPVNAVDNSGKTALMMAAENGQAGAVDILVNSAQADLTVKDKDLNTPL HLACSKGHEKCALLILDKIQDESLINEKNNALQTPLHVAARNGLKVVVEELLAKGACVLAVDENASRSNG PRSTPGTAVQKEE TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin