DESCRIPTION

• Tag: Tag Free
• Accn: BC059039
• Type: Mouse Untagged Clone
• Refseq: BC059039, AAH59039
• Status: In Stock*
• Symbol: Magee1
• Vector: PCMV6-Kan/Neo
• Category: cDNA Clones
• Locus Id: 107528
• Synonyms: mMage-e1, DAMAGE
• Refseq Orf: 2757 bp
• Insert Size: 2757 bp
• Refseq Size: 3532 bp
• Availability: In Stock
• Cytogenetics: X D
• Gene Summary: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (By similarity).[UniProtKB/Swiss-Prot Function]
• Sequence Data: Fully Sequenced ORF ( show hide ) >BC059039 GGCGAGCATTCTGAGACTAGCGTTCACAGTCGGCTAGAACCTTCGTTCTTGCCCACCACCTCTAATTTCT GCTCCTGCGACTTGCTCGGGCCTGGCAGCAGCAGCATTCCTGCTCTAGCGGCAGTTCCGTGCAGAGGCGC CTACATACCTCTCCCCCTCGTCTGTGAGATCGCGGGCCTGTCAGTGTCAGCTTCCACACGTCGGTCGACA GGACCATGTCGCTGGTAAGCCAGAATTCGCGCCGCCGCCGCGGTGGAAGGGCCAATGCGCGCAGAAACAA CGGGAAGGGTCACCCTGCTGCTGTGCCAGGCCCAGACGTCCCTCGGGATCGCAACGATCCTAAGATCCTC CAGGGCCTCCGCGCCTCTGAGGGCCCTGGCACCTCCATGCTGCCCACCCCCCGGGAGGGCCCAAGCGCCT CTGTGCCGCCCACCGCCTCAGAGGGCTCAAGCGCCCCTAGGCAGTTCATCATTTCCCAGGGGCCGAACAC CTCCGAGATGCCCACCTCCAGGAAGGGTCGGGGCGCCTCCCGGCCTCCTGCAGTTTCCGCAGGGCTGAAC ACCGCTATGTCAATCACCGCCTCGGAGGGCCCGAATAGCCCTGTGCCGCCCACTGCTCCTAAAGGCTCAA AGGCCTATGAGCATCTCCCTGTCTCAGAAGGGCTGGCCATCTCTGAGCAGCGTCACTCTGATGGGGGCCC TAACATGGAGCCCACCCTGGGTGAGGGCCCGGGAATCTCGGTGCCGCCCACCTTCTCTGAGGAATCGGGC ATTTCTGATGAGGGCTTGAGCATCTTTATGTCGCCCAACATCTCGGAGGGACCGGGTATCAACGAGCCCT ACTCCGTTAGTGAGGACCCAAGCACTTCGGTGCCGCCCACTGACTCTAATGGACTGGGCATCAACCTGCC GCCCACCTTCGGTGAGGGCCTGAGCATCTCTATGCTGTTCTCCGCTTTGGAGGAACCCGACATCTTTGCG CCGCCCCCCTCCGCTGAGGGACTGTTCGCCTCCATGTCGCCCCCCTCCGGTGAGATCCAGAGCTCCTGGG TGTCGCCCATCATAATGGAGGGATGTAACGTCAACGTGCCGCCCACCTCCAAAAAGGGACTGCGCACCTC GGTGCCATCAGCTGCTTGTGAGAGCCCGAGCACCTCAGCCGAGGGCCTGAGCTCGTCCCTGTCATCCATT TCTGCTGAGGGCTTCTGCAGCTCGCTGGCGCCATGCGCTGCGGAGGGATCCTGTGAGCTGCTTCCCTGCG GGGAGGGCCGGAGCACCTCGGAGCTGCACTGCCTGGGGGAGGGGTCGAGCACCTCGCAGATGTCCCTAGC TGCCGAGGGCCCTAGCGCTTCTGGGATGCCCACTGAAGCTAATAACCCCGAGGAAGCACTGAGCTGCTGT GCTTCTGAGCGCAGGAACAAGAGCACCTCCCGTGCTCTGCAAAAGGCCAAGGATCCATCTGTACGCCCAA AGCGTGAAGACCGCTTCCTGGATTTCCAGGTCCTGAGAGACAGTAAGAATTCCAACTCCATCACCATTAT GGGCCTGGGCACCTCCCGCGTTGCTCTTACCTTGAAGCCTCAGGATCCCATGGAGCAAAACGTAGCAGAA TTGTTGCAGTTTCTGCTGCTGAAGGATCAGACCAAGTACCCTATCAAGGAATCTGATATGAGGGAATTCA TTGACAAAGACTATCGCCACCAGTTCCCGGAGATCCTCAGACGAGCAGCAGTCCACCTGGAGTGCATTTT TCGGTTTGAACTGAAGGAGCTCGACACTGAGGAGCACATCTACATCCTGCTCAACAAACTGGGACCAGTG CCCTTTGAAGGGTTAGAAGACGTCCCAAATGGGCCAAAGATGGGCCTCTTGATGATGATTCTGGGACACA TATTACTAAATGGCAACCAAGCGAGAGAAGCTGATATCTGGGAGATGCTCTGGAGATTCGGAGTGCAGCG TGAAAGAAGGCTTTCTGTTTTTGGGAACGTGAAGAGACTTCTGTCTGTAGAGTTTGTGTGGCAGCGTTAC TTGGACTATAGGCCACTAACTGACTGTGTGCCAGTCGAGTATGAGTTTTACTGGGGCCCACGATCCCGCG CAGAAACCACCAAGATGAAAATTCTGAAGTTCATGGCTAAGATCTATAACAAAGATCCTATGGACTGGCC AGCCCTGTACAACGAAGCTCTGGAAGAAGACGCTGACAGAGTTGTTGTTAATAACTTCAGAGTTGCTCGT CCATTTAGGAGGCCCCTTTTTGCAGAAGTTGCTCCAGAGCTAGATGCTTCTGGCTCAAAATATTCTCCCC ATTCTTGGCCTGAGTCAAGATTAGAGAGCAAGGCAAGGAAATTGGTCCAGTTATTTCTGCTGATGGATTC AACTAAGCTGCCTATACCAAAGAAGGGAATCCTGTATTACATCGGTCGAGAGTGCACCAAAGTGTTCCCT GATCTCCTGAATCGTGCTGCTCGCACCCTAAACCACGTGTATGGCACAGAGCTAGTGGTCCTTGATCCCA GGAACCACTCCTACACCCTGTACAACCGAAGAGAAATGGAAGATACAGAAGAGATCATGGACAGTCCAAA CAGGCCCGGCAACAATTTCTTAATGCAGGTTCTGAGCTTCATCTTTATCATGGGCAACCATGCGAGGGAG TCTGCAGTCTGGGCCTTTCTAAGGGGCTTGGGAGTTCAAAATGGCAGAAAGCACGTGATTACCTGTCGGT ATTTGAGTCAGCGCTACCTAGACAGTTTGCGGGTTCCTGACAGTGATCCGGTGCAATATGATTTTGTATG GGGCCCTAGAGCCCGCCTGGAAACCTCCAAGATGAAAGCCCTGCGATATGTGGCCCGAATCCACAGAAAG GAGCCAGAAGACTGGCCGGAGCAGTACAGGGAGGCAATGGAAGATGAGGCCAACAGAGCTGAAGCTGGGC GTCGTCCGTTGATCGTTCGCAACTTGCGATAGATTGTGTCATCGAGGCCTTGTATGGCCAGGCCTAGAGC TCTGGTTCTCATGTATTAGGAGTGGGTGTGTGTGTGTGTTGGGGGGGGGGTACGTATTGTATTTGATACT TGTGTTCCAGTTCCACTTATGTATCTTTTCATATTTAGTTCTTGTTTGGTATCTTGAAAAGTTTCCATTG TTTTAGTAATACTGTCCATTAATGTAAATGTGATTGACCACTTACTGTCTCTCTGGTGTACTTTGTGCTG TGAGCATTTTGATAGTGTTATAAAGTGTTTTAGGACTCTTTCCATCTTGTTGCATTGTCTGAAAGAGAAC ATATAGCAGATAGCTATAGATATAGGCTTTTCCTTGAAAGCTTGAAAAAAAATCATCAGTAAAGTGATCT AGTTTCAGATGTTAAACAAATATAGAACAACTGACAAAGTACTAAGTTGTGGCTGGCTTCCCTTTTGTCT GCTATTGTGTAAAGAATACTAACGTTTCCCTGTATTTGCTTTTGCTGTACTAAAGTGTGTTGAAAAATAA AAGTGTAATAAATCAAAAAAAAAAAAAAAAAA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: AscI-NotI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin