DESCRIPTION

• Aa: 1-414(end)
• Mw: 47.6 kDa
• Tags: C-terminal FLAG-tag
• Format: Aqueous buffer solution
• Genbank: NM_005896
• Species: Human
• Uniprot: O75874
• Shiptemp: -80°C (dry ice)
• Synonyms: Isocitrate Dehydrogenase 1, IDH1, oxalosuccinate decarboxylase
• Warnings: Avoid freeze/thaw cycles.
• Category: Metabolic Enzymes/Protein
• References: 1. Dang, L., et al. (2010). Trends Mol. Med. 16(9):387-97. 2. Paschka, P., et al. (2010). J. Clin. Oncol. 28(22):3636-43.3. Fu, Y., et al. (2010). Biochem. Biophys. Res. Commun. 397(2):127-30.
• Description: Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, GenBank Accession No. NM_005896, full length [a.a. 1-414(end)] with C-terminal FLAG-tag, MW= 47.6 kDa, expressed in Sf9 cells via a baculovirus expression system.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 3 mM DTT and 10% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction was conducted_x000D_ in 50 mM Tris (pH 7.4), 10 mM MgCl2, 200_x000D_ µM DL-isocitrate, and 200 µM NADP._x000D_ Incubate for 30 minutes at room_x000D_ temperature. Read at Abs340 using Tecan_x000D_ Infinite 1000 (5 flash/10 ms settle time).
• Specific Activity: ≥5600 pmol/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Metabolic Enzymes-Dehydrogenase