DESCRIPTION

• Category: ZYMO KITS
• Keywords: D3021, Z5122
• Paragraph 1: The Quick-DNA Microprep Kit is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources.  This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples.
• Paragraph 2: For processing, simply add the specially formulated Genomic Lysis Buffer to a sample, vortex, and transfer the mixture to the supplied Zymo-Spin™ Column.  There is no need for organic denaturants or Proteinase K digestion because of the unique lysis buffer system.  The product features Zymo-Spin™ technology to yield high-quality, purified DNA in just minutes (see below).  PCR inhibitors are effectively removed during the purification process.  DNA purified using the Quick-gDNA™ MicroPrep is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
• Bullet Point 1: Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells in less than 15
• Bullet Point 2: Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
• Bullet Point 3: Unique extraction technology excludes the use of Proteinase K and organic denaturants.
• Bullet Point 4: Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
• Bullet Point 5: DNA Recovery: Up to 5 µg total DNA is eluted into ?10 µl (6 µl minimum) DNA Elution Buffer or water.
• Bullet Point 6: Human whole blood will typically yield 1.5-3.5 µg DNA per 50 µl blood sampled.
• Bullet Point 7:  Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues.
• Bullet Point 8:  3-5 µg DNA per mg liver, kidney and lung tissues.
• Manufacturer Sku: D3021