DESCRIPTION

• Form: Antigen Affinity Purified from Pooled Serum
• Gene: PLK1
• Host: Rabbit
• Rrid: AB_2492209
• Type: Primary Antibody
• Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Target: Polo-Like Kinase (Thr210)
• Isotype: IgG
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Shipping: Blue Ice
• Synonyms: Cell cycle regulated protein kinase antibody, PLK 1 antibody, PLK antibody, PLK-1 antibody, plk1 antibody, PLK1_HUMAN antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, Serine/threonine protein kinase PLK1 antibody, Serine/threonine-protein kinase 13 antibody, Serine/threonine-protein kinase PLK1 antibody, STPK 13 antibody, STPK13 antibody
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 of human PLK1, conjugated to keyhole limpet hemocyanin (KLH).
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~66 kDa PLK phosphorylated at Thr210. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
• Applications: WB
• Ncbi Gene Id: 5347
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 66
• Production Notes: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Dilution Range Wb: 1:1000
• Immunogen Species: Human
• Species Reactivity: Human and Rat
• Target Description: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser-137 and Thr-210 (van de Weerdt et al., 2005).
• Expected Reactivity: Bovine, Canine, Non-Human Primate, Mouse, Xenopus, Zebrafish
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: P53350
• Post Translational Modification Type: Phosphorylated
• Post Translational Modification Residue: Thr210

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