Anti-CREB (N-terminal region) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CREB1
Host: Rabbit
Rrid: AB_10837377
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: CREB (N-terminal region)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Cyclic AMP, CREB
Clonality: Rabbit Polyclonal
Immunogen: CREB synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human CREB. This sequence is well conserved in rat, mouse, and chicken CREB, and has low homology to other CREB family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, HeLa, and rat PC12 cells.
Applications: WB, ICC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 43
Dilution Range Ip: 1:100
Dilution Range Wb: 1:500
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in the nervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calcium influx and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271 potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation for CREB-dependent transcription in response to genotoxic stress.
Uniprot Number Immunogen Species: P16220