Aflatoxin Q1, a hydroxy metabolite of Aflatoxin B1 (AFB1)—a mycotoxin synthesized by Aspergillus flavus (A. flavus)—and aflatoxin B1 8,9-oxide are principal oxidative derivatives produced from Aflatoxin B1 in human liver microsomes across all substrate concentrations. The conversion of Aflatoxin B1 to Aflatoxin Q1 through 3 alpha-hydroxylation represents a critical detoxification route [1] [2].