DESCRIPTION

• Notes: Avoid exposing to excessive heat or light. Upon first thaw, prepare single-use aliquots and store at -20°C. Luciferin is very sensitive to multiple freeze/thaw cycles. Discard any unused product after thawing from aliquots.
• Shiptemp: -20°C
• Warnings: Avoid freeze/thaw cycles. Protect from light.
• Category: Cell Signaling Pathway/Buffer
• Background: Luciferases are enzymes that produce light when they oxidize their substrate. Oxidation of luciferin, the substrate for the most commonly used firefly (Photinus pyralis) luciferase, results in bioluminescence that can be accurately measured using a luminometer and is proportional to the amount of luciferase enzyme present in the system under study. The firefly luciferase reaction requires luciferin, plus adenosine triphosphate (ATP) and Mg2+ as cofactors, and when in the presence of O2, emits a characteristic glow (Figure 1). Common biological experiments that utilize the luciferase reaction are promoter activity assays in which luciferase expression is under the control of a specific promoter, and cell line-derived xenograft (CDX) models in which constitutive luciferase expression allows for real-time, non-invasive monitoring of tumor growth and measurement of disease progression or drug efficacy._x000D_ _x000D_ Figure 1. Bioluminescent Reaction Catalyzed by Luciferase.
• Description: The In vivo-Luc™ imaging solution is a ready-to-use, sterilized, endotoxin-free solution of D-Luciferin for in vivo imaging of the expression of firefly luciferase. Real-time disease progress or drug efficacy in animals can be monitored in a non-invasive manner using the In vivo-Luc™ imaging solution.
• Formulation: 15 mg/ml D-Luciferin in Dulbecco's phosphate-buffered saline (DPBS) sterilized and endotoxin free.
• Unspsc Code: 41116155
• Unspsc Name: Molecular biology and cell culture growth media
• Applications: In vivo imaging of luciferase activity
• Product Type: Buffers and Biochemical Reagents
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 60650, 60683, 78409, 78494
• Storage Stability: Reagents will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. Upon first thaw, prepare single-use aliquots and store at -20°C. Luciferin is very sensitive to multiple freeze/thaw cycles. Discard any unused product after thawing from aliquots. 
• Scientific Category: Cell Signaling Pathway