DESCRIPTION

• Form: Lyophilized powder
• Host: Rabbit
• Clone: NA
• Label: ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.
• Purity: Affinity purified on immobilized antigen.
• Target: Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 162514
• Isotype: Rabbit IgG
• Homology: Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical
• Is Toxin: No
• Sequence: (C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3
• Synonyms: Transient receptor potential cation channel subfamily V member 3, Vanilloid receptor-like 3, VRL3
• Accession: Q8NET8
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Human|Rat|Mouse
• Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
• Applications: FC|IC|IF|IHC|LCI
• Preservative: 1% BSA, 0.05% NaN3
• Reconstitution: 50 µl double distilled water (DDW).
• Blocking Peptide: BLP-CC033
• Negative Control: BLP-CC033
• Positive Control: NA
• Immunogen Location: 1st extracellular loop
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Human
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: FC|IC|IF|IHC|LCI
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (52°C).6,7TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.8
• Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #ACC-033), and immunohistochemistry (labeled antibody).
• Application Dilutions Western Blot Wb: Contact Alomone for information
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information