2-1042-025
10x Buffer BXT; Strep-Tactin®XT Elution Buffer
IBA Lifesciences
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DESCRIPTION
10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer. It has to be diluted before use. Biotin reversibly binds to Strep-Tactin®XT, which allows the elution of Strep-tag®II or Twin-Strep-tag® fusion proteins. Please note that biotin binds irreversibly to Strep-Tactin®. Therefore, 10x Buffer BXT is not applicable for elution of Strep-tag®II or Twin-Strep-tag® proteins from Strep-Tactin® resins, since they cannot be regenerated after use.
DETAILS
- Ph: 8.0
- Category: Buffers & Biochemicals
- Hazardous: No
- Lead Time: 3-5 days
- Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins
- Composition: 1 M Tris-Cl, 1.5 M NaCl, 10 mM EDTA, 500 mM biotin, pH 8