DESCRIPTION

• Aa: 2-end (all components)
• Mw: 325 kDa (complex)
• Tags: N-terminal FLAG (EED), N-terminal His-tag (rest)
• Notes: *This mutation is equivalent to R679C in variant 3 (GenBank Accession No. NM_001203247).
• Format: Aqueous buffer solution
• Genbank: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
• Species: Human
• Uniprot: Q15910
• Mutation: R679C
• Shiptemp: -80°C (dry ice)
• Synonyms: KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, RBAP48, Retinoblastoma Binding Protein 4 (RBBP4), hEED, Suppressor Of Zeste 12 Homolog, CHET9, JJAZ1; AE binding protein 2, Adipocyte Enhancer binding protein 2, EZH
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Enzymes
• Background: EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
• Description: Mutant version of BPS's regular EZH2 5-member complex (Cat. # 51004), but with an Arg-to-Cys mutation on a.a. 684 of the EZH2 protein.* Complex of human EZH2 (GenBank Accession No. NM_004456), (a.a. 2-end ; R684C) with N-terminal His-tag, MW= 86kDa, human EED (NM_003797) (a.a. 2-end) with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355) (a.a. 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a.a. 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a.a. 2-end) with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system. *This mutation is equivalent to R679C in variant 3 (GenBank Accession No. NM_001203247).
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
• Supplied As: aqueous buffer solution
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex.
• Host Species: insect
• Product Type: Proteins
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Methyltransferase