Firefly Luciferase-eGFP Lentivirus (Puromycin)-79980-P

DESCRIPTION

The Firefly Luciferase-eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) driven by a CMV promoter. Both the luciferase and eGFP are coexpressed under the CMV promoter in the transduced cells, allowing greater flexibility for detection of transduced cells.

DETAILS

Notes: License Disclosure: Visit bpsbioscience.com/license for the label license and other key information about this product.
Species: Replication incompetent HIV
Shiptemp: -80°C (dry ice)
Synonyms: pseudotyped lentivirus, pseudovirus, pseudovirion, 79980-G, 79980-P
Warnings: Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Category: Cell Signaling/Lentivirus
Description: The Firefly Luciferase-eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) driven by a CMV promoter. Both the luciferase and eGFP are coexpressed under the CMV promoter in the transduced cells, allowing greater flexibility for detection of transduced cells.
Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
Supplied As: Two vials (500 µl x 2) of Firefly Luciferase-eGFP lentivirus at a titer ≥1 x 10^7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Applications: Ideal as a positive control for transduction; useful for transduction optimization.
Product Type: Lentivirus
Biosafety Level: BSL-2
Related Products: 60690, 60187, 79942, 79981, 79982
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Scientific Category: Cell Signaling Pathway
Instructions for Use: See assay protocol for detailed instructions.