SK3041
Better Bradford Protein Assay Kit (Improved linearity), 1000preps
Bio Basic
DESCRIPTION
Better Bradford Protein Assay Kit: The Better Bradford Protein Assay Kit is a quick and ready-to-use coomassie-binding, colorimetric method for total protein quantitation. This modification of the well-known Bradford method greatly reduces the tendency of coomassie reagents to give nonlinear response curves by a formulation that substantially improves linearity for a defined range of protein concentration. In addition, the Kit results in significantly less protein-to-protein variation than is observed with other Bradford-type coomassie formulations. When coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Performing the assay in either test tube or microplate format is simple: Combine a small amount of protein sample with the assay reagent, mix well, incubate briefly and measure the absorbance at 595 nm. Protein concentrations are estimated by reference to absorbance obtained from a series of standard protein dilutions, which are assayed alongside the unknown samples.
DETAILS
- Sap Code: C503041-1000
- Unspsc Code: 41116012
- Bbi Code Sku: SK3041.SIZE.1000Assays
- Unspsc Category: Protein Assays
- Storage Conditions: (-15 to -20)C
- Tags Search Keywords: Protein - Quantitation Kits; Assay - Bradford Modified (Improved linearity); Bradford Protein Assay Kit; Modified; Biobasic; Bio Basic; Bio Basics; Biobasics
- Product Category Level 1: Molecular Biology Kits
- Product Category Level 2: Protein - Assays
- Product Category Level 3: Bradford
- Shipping Conditions Rtice: ICE
- Refrigeration Requirements: Freezer