Single Step Ultra Competent Cell Preps Kit: This Kit contains SSCS soluon, enables researchers to prepare competent E. coli cells in a single step and to transform the cells without heat‐shock. SSCS has been reported to be faster and easier than other methods of producing competent cells, such as the tradional CaCl2 method described by Sambrook et al, or other high competency protocols. The competent cells obtained by this method can be kept at ‐70°C for long term storage. Transformaon efficiencies depend on the strain of E. coli, as well as the nature and quality of the transforming DNA.