DESCRIPTION

• Aa: 2091-2490
• Mw: 71.8 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_080685
• Species: Human
• Uniprot: Q12923
• Shiptemp: -80°C (dry ice)
• Synonyms: Bas, FAP-1, PTPN13, APO-1/CD95-Associated Phosphatase 1, PTP-BAS, PNP1, PTP1E, PTPL1, PTPLE, PTP-BL, Fas-Associated Phosphatase 1
• Warnings: Avoid freeze/thaw cycles.
• Category: Protein Phosphatase/Non-Receptor
• Background: PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN13 is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. PTPN13 binds to a negative regulatory domain in Fas that inhibits Fas-induced apoptosis.
• References: 1. W. Kimber, et al., Biochem.J. 2003, 376: 525-535. 2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.
• Description: Human, recombinant PTP-Bas, catalytic domain (Gene bank accession number NM_080685), a.a. 2091-2490 (catalytic domain), with N-terminal GST tag, MW=71.8 kDa, expressed in an E. coli expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
• Assay Conditions: The specific activity of Bas was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.
• Specific Activity: 5 U/µg
• Storage Stability: >12 months at -80°C
• Scientific Category: PTP/Non-rec.