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PTE-HIF1A-P402-T-1 PTE-HIF1A-P402-T-2 PTE-HIF1A-P402-T-5

Human Hydroxylated-HIF-1 alpha (P402) and HIF-1 alpha ELISA



RayBio® Human Hydroxylated-HIF-1 alpha (P402) ELISA Kit. This ELISA is for measuring hydroxylated HIF-1 alpha (Pro-402) as well as HIF-1 alpha in human cell lysates.


  • Storage: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
  • Category: ELISA
  • Lead Time: 4 weeks
  • Assay Type: Sandwich
  • Sample Type: Cell Lysates, Tissue Lysates
  • Specificity: The antibody pair provided in this kit recognizes human hydroxylated HIF-1 alpha hydroxylated at site Proline-402 as well as Hif-1 alpha.
  • Subcategory: Post-Translational Modification
  • Result Output: Semi-Quantitative
  • Accession Number: Q16665
  • Detection Method: Colorimetric
  • Species Reactivity: Human