DESCRIPTION

• Notes: The AAV-DJ viruses are covered under several patents, including U.S. Patent Nos. 7,588,772, 8,067,014, 8,574,583, and 8,906,387, as well as corresponding foreign patents applications and patent rights. AAV-DJ is used under a license agreement. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com. Biosafety: Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell's genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions.
• Species: AAV-DJ
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles
• Category: CART/AAV
• Background: Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications allow the AAV-DJ serotype to exhibit improved transduction efficiency in vitro and in vivo and infect a broader range of cell types compared to the wild-type serotypes. Telomerase reverse transcriptase (TERT) plays a key role in cancer formation, ensuring chromosomal stability by maintaining telomere length, and allowing cells to avert senescence. It constitutes a limiting factor for formation of the telomerase complex in cancer cells. The human TERT promoter favors transgene expression in cancer cells.
• Description: These AAV-DJ particles constitutively express the firefly (Photinus pyralis) luciferase under the control of a TERT promoter.
• Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68. Virus particles can be packaged in custom formulations by special request, for an additional fee.
• Supplied As: Two vials (50 µl x 2) of AAV at a titer ?1 x 1012 vector genomes/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: Positive control in the transduction of cancer cells. Optimization of transduction assays and tracking of transgene expression over time.
• Product Type: AAV virus
• Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium #21005-1L). The purity varies with each lot; the exact value will be provided with each shipment.
• Biosafety Level: BSL-1
• Related Products: 78469, 78478, 78460
• Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
• Scientific Category: Cell Signaling Pathway
• Signed License Required: No