DESCRIPTION

• Form: Antigen Affinity Purified from Pooled Serum
• Gene: EEF1A2
• Host: Rabbit
• Rrid: AB_2492084
• Type: Primary Antibody
• Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Target: eEF1A2 (Ser358)
• Isotype: IgG
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Shipping: Blue Ice
• Synonyms: eEF1A-2 antibody, Eef1a2 antibody, EEF1AL antibody, EF 1 alpha 2 antibody, EF-1-alpha-2 antibody, EF1A antibody, EF1A2_HUMAN antibody, Elongation factor 1 A 2 antibody, Elongation factor 1 alpha antibody, Elongation factor 1 alpha 2 antibody, Elongation factor 1-alpha 2 antibody, Eukaryotic elongation factor 1 A-2 antibody, Eukaryotic translation elongation factor 1 alpha 2 antibody, FLJ41696 antibody, HS1 antibody, OTTHUMP00000031776 antibody, Statin like antibody, Statin S1 antibody, Statin-S1 antibody, STN antibody, STNL antibody
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2, conjugated to keyhole limpet hemocyanin (KLH).
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~50 kDa eEF1A2 phosphorylated at Ser358. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
• Applications: WB
• Ncbi Gene Id: 1917
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 50
• Production Notes: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Dilution Range Wb: 1:1000
• Immunogen Species: Human
• Species Reactivity: Human and Mouse
• Target Description: Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser-358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013).
• Expected Reactivity: Chicken, Non-Human Primate, Rat, Sheep, Xenopus
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: Q05639
• Post Translational Modification Type: Phosphorylated
• Post Translational Modification Residue: Ser358

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