DESCRIPTION

• Aa: 35-242
• Mw: 52.5 kDa
• Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
• Notes: HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.
• Format: Aqueous buffer solution
• Genbank: NM_005191
• Species: Human
• Uniprot: P33681
• Shiptemp: -80°C (dry ice)
• Synonyms: CTLA-4 Counter-Receptor B7.1
• Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
• Category: Immunotherapy/Protein
• References: 1. Byrum, J.N., Van Komen, J.S., Rodgers, W., J. Immunol. 2013 Sep 15;191(6):3073-3081. 2. Legat, A., et al., Front Immunol. 2013 Dec 19;4:455.
• Description: Human secreted B7-1-Fc fusion protein, also known as CD80, with C-terminal Avi-tag™. GenBank Accession No. NM_005191, a.a 35-242 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 52.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
• Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Reaction done according to CTLA4:B7-1[biotin] assay kit (Catalog # 72009) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added ON at 4 deg 100 ng/well. Various concentrations of biotinylated ligand (0-8ng/ul, 0.5 ng/ul is the recommended kit condition) added for 2h at RT. Strep-HRP added for 1h at RT.
• Related Products: 71127, 71125, 79572, 79512
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Immunotherapy