DESCRIPTION

• Aa: 2-end
• Mw: 119 kDa
• Tags: N-terminal FLAG-tag
• Format: Aqueous buffer solution
• Genbank: NM_003334
• Species: Human
• Uniprot: P22314
• Shiptemp: -80°C (dry ice)
• Synonyms: UBE1, UBA1
• Warnings: Avoid freeze/thaw cycles.
• Category: Ubiquitination/Activating Enzyme (E1)
• References: 1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008).2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).
• Description: Human UBE1 (GenBank Accession No. NM_003334), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assay was done according to UBE1 Inhibitor Screening Assay Kit (BPS Bioscience #79957) with UBE1 titrated from 1500 ng/rxn to 0 ng/rxn.
• Specific Activity: 5 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Ubiquitin Activating Enzyme (E1)