mouse monoclonal IgG2a; Luciferase Antibody (C-12) is an IgG2a κ mouse monoclonal Luciferase antibody (also designated Firefly Luciferase antibody) that detects the Luciferase protein of Photinus pyralis origin by WB, IP, IF and ELISA. Luciferase Antibody (C-12) is available as both the non-conjugated anti-Luciferase antibody form, as well as multiple conjugated forms of anti-Luciferase antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Luciferase isolated from the common North American firefly, Photinus pyralis, is one of the most extensively studied enzymes that catalyze light production in bioluminescent organisms. Luciferase belongs to the ATP-dependent AMP-binding enzyme family. It produces green light with a wavelength of 562 nM. Following is the chemical catalytic reaction, which is catalyzed by Luciferase: Photinus luciferin + O(2) + ATP = oxidized Photinus luciferin + CO(2) + AMP + diphosphate + light