Anti-Phosphotyrosine Antibody

DETAILS

Form: Protein A Purified
Gene: N/A
Host: Mouse
Rrid: AB_1944435
Type: Primary Antibody
Clone: M375
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Anti-Phosphotyrosine
Isotype: IgG2b
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Phosphotyrosine mAb
Clonality: Mouse Monoclonal
Immunogen: Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
Applications: WB, ICC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: N/A
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
Uniprot Number Immunogen Species: N/A
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: p-Tyr