Rat CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

DESCRIPTION

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

DETAILS

Tag: C-10×His | C-Flag
Host: HEK293
Size: 100ug
Buffer: PBS, pH7.4
Format: Powder
Purity: 90%
Source: Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-R52D8) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Asp 103 (CD3E) & Phe 22 - Gly 106 (CD3D) (Accession # D4A5M2-1 (CD3E) & P19377-1 (CD3D)).
Storage: -20℃
Category: Bio-Markers & CD Antigens
Molecule: CD3E & CD3D
Accession: NP_001101610.1 | NP_037301.1
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Gln 21 - Asp 103 (CD3E) & Phe 22 - Gly 106 (CD3D)
Swiss-prot: D4A5M2-1 (CD3E) & P19377-1 (CD3D)
Package Size: 100ug*1
Characteristics: Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 14.9 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 19-20 kDa and 27-33 kDa under reducing (R) condition, and 37-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 14.9 kDa | 14.5 kDa
Shipping Condition: RT
Species Reactivity: Rat
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