Monoclonal Antibody to CD45RO (T-Cell Marker)(Clone : UCHL-1)

DESCRIPTION

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

DETAILS

Gene: PTPRC
App Id: FACS,IF,IHC
Antigen: CD45RO (T-Cell Marker)
Gene Id: 5788
Isotype: Mouse IgG2a, kappa
Format Id: Purified
Clone Name: UCHL-1
Uniprot Id: P08575
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: PTPRC||CD45
Application Note: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Cultured human T-cells from an IL-2-dependent T-cell line (CA1)