Anti-NLRC4 (Ser-533), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: NLRC4
Host: Rat
Rrid: AB_3068479
Type: Primary Antibody
Clone: M549
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: NLRC4 (Ser-533)
Isotype: N.T.
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: CARD12, CLAN1, IPAF, NLR family CARD domain-containing protein 4, NOD-like receptor 4
Clonality: Rat Monoclonal
Immunogen: Clone M549 was generated from a phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide, but does not detect this peptide after lambda phosphatase treatment.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 110
Dilution Range Wb: 1:500
Immunogen Species: Mouse
Species Reactivity: Human, Mouse, and Rat
Target Description: The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family is a diverse family of cytoplasmic innate immune receptors that are involved in recognition of pathogen-associated molecular patterns. NLRs are important for pathogen sensing, transcriptional activation of proinflammatory cytokines and activation of inflammatory caspases. NLRC4 (IPAF, CARD12) forms the inflammasome that responds to bacterial flagellin. This inflammasome is activated by NLRC4 oligomerization, NAIP protein binding, and activation of caspase-1 leading to pyroptosis. NLRC4 is phosphorylated on Ser-533 by PKCδ following infection of macrophages with S. typhimurium. Mutant forms of NLRC4 demonstrate that an unphosphorylatable form (S533A) does not activate caspase-1 and pyroptosis in response to S. typhimurium, while a phosphomimetic NLRC4 (S533D) mutant causes rapid macrophage pyroptosis without infection. Thus, PKCδ phosphorylation of NLRC4 (S533) may be a critical event in inflammasome activation and host innate immunity.
Uniprot Number Immunogen Species: Q3UP24
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-533