DESCRIPTION

• Mw: 70 kDa
• Tag: Myc-DDK
• Accn: NM_001130675
• Type: Human Tagged ORF Clone
• Refseq: NM_001130675.1, NP_001124147.1
• Status: In Stock*
• Symbol: Calmegin
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 1047
• Orf Size: 1830 bp
• Refseq Orf: 1833 bp
• Uniprot Id: O14967
• Refseq Size: 2828 bp
• Availability: In Stock
• Cytogenetics: 4q31.1
• Gene Summary: Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq, Jul 2008]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC226016 ORF sequence Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGCATTTCCAAGCCTTTTGGCTATGTTTGGGTCTTCTGTTCATCTCAATTAATGCAGAATTTATGGATG ATGATGTTGAGACGGAAGACTTTGAAGAAAATTCAGAAGAAATTGATGTTAATGAAAGTGAACTTTCCTC AGAGATTAAATATAAGACACCTCAACCTATAGGAGAAGTATATTTTGCAGAAACTTTTGATAGTGGAAGG TTGGCTGGATGGGTCTTATCAAAAGCAAAGAAAGATGACATGGATGAGGAAATTTCAATATACGATGGAA GATGGGAAATTGAAGAGTTGAAAGAAAACCAGGTACCTGGTGACAGAGGACTGGTATTAAAATCTAGAGC AAAGCATCATGCAATATCTGCTGTATTAGCAAAACCATTCATTTTTGCTGATAAACCCTTGATAGTTCAA TATGAAGTAAATTTTCAAGATGGTATTGATTGTGGAGGTGCATACATTAAACTCCTAGCAGACACTGATG ATTTGATTCTGGAAAACTTTTATGATAAAACATCCTATATCATTATGTTTGGACCAGATAAATGTGGAGA AGATTATAAACTTCATTTTATCTTCAGACATAAACATCCCAAAACTGGAGTTTTCGAAGAGAAACATGCC AAACCTCCAGATGTAGACCTTAAAAAGTTCTTTACAGACAGGAAGACTCATCTTTATACCCTTGTGATGA ATCCAGATGACACATTTGAGGTGTTAGTTGATCAAACAGTTGTAAACAAAGGAAGCCTCCTAGAGGATGT GGTTCCTCCTATCAAACCTCCCAAAGAAATTGAAGATCCCAATGATAAAAAACCTGAGGAATGGGATGAA AGAGCAAAAATTCCTGATCCTTCTGCCGTCAAACCAGAAGACTGGGATGAAAGTGAACCTGCCCAAATAG AAGATTCAAGTGTTGTTAAACCTGCTGGCTGGCTTGATGATGAACCAAAATTTATCCCTGATCCTAATGC TGAAAAACCTGATGACTGGAATGAAGACACGGATGGAGAATGGGAGGCACCTCAGATTCTTAATCCAGCA TGTCGGATTGGGTGTGGTGAGTGGAAACCTCCCATGATAGATAACCCAAAATACAAAGGAGTATGGAGAC CTCCACTGGTCGATAATCCTAACTATCAGGGAATCTGGAGTCCTCGAAAAATTCCTAATCCAGATTATTT CGAAGATGATCATCCATTTCTTCTGACTTCTTTCAGTGCTCTTGGTTTAGAGCTTTGGTCTATGACCTCT GATATCTACTTTGATAATTTTATTATCTGTTCGGAAAAGGAAGTAGCAGATCACTGGGCTGCAGATGGTT GGAGATGGAAAATAATGATAGCAAATGCTAATAAGCCTGGTGTATTAAAACAGTTAATGGCAGCTGCTGA AGGGCACCCATGGCTTTGGTTGATTTATCTTGTGACAGCAGGAGTGCCAATAGCATTAATTACTTCATTT TGTTGGCCAAGAAAAGTAAAGAAAAAACATAAAGATACAGAGTATAAAAAAACCGACATATGTATACCAC AAACAAAAGGAGTACTAGAGCAAGAAGAAAAGGAAGAGAAAGCAGCCCTGGAAAAACCAATGGACCTGGA AGAGGAAAAAAAGCAAAATGATGGTGAAATGCTTGAAAAAGAAGAGGAAAGTGAACCTGAGGAAAAGAGT GAAGAAGAAATTGAAATCATAGAAGGGCAAGAAGAAAGTAATCAATCAAATAAGTCTGGGTCAGAGGATG AGATGAAAGAAGCAGATGAGAGCACAGGATCTGGAGATGGGCCGATAAAGTCAGTACGCAAAAGAAGAGT ACGAAAGGAC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC226016 protein sequence Red =Cloning site Green =Tags(s) MHFQAFWLCLGLLFISINAEFMDDDVETEDFEENSEEIDVNESELSSEIKYKTPQPIGEVYFAETFDSGR LAGWVLSKAKKDDMDEEISIYDGRWEIEELKENQVPGDRGLVLKSRAKHHAISAVLAKPFIFADKPLIVQ YEVNFQDGIDCGGAYIKLLADTDDLILENFYDKTSYIIMFGPDKCGEDYKLHFIFRHKHPKTGVFEEKHA KPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDE RAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPA CRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTS DIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSF CWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKS EEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Transmembrane
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin