mouse monoclonal IgG1; CAF-1 p60 Antibody (B-10) is an IgG1 κ mouse monoclonal CAF-1 p60 antibody that detects CAF-1 p60 of mouse, rat and human origin by WB, IP, IF and ELISA. CAF-1 p60 Antibody (B-10) is available as the non-conjugated anti-CAF-1 p60 antibody form, as well as multiple conjugated forms of anti-CAF-1 p60 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.