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ALR-033_25 mcl ALR-033_50 mcl ALR-033_0.2 ml ALR-033-CF_0.2 ml

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Anti-LPAR3 (EDG7) (extracellular) Antibody

Alomone Labs

DESCRIPTION

Anti-LPAR3 (EDG7) (extracellular) Antibody (#ALR-033) is a highly specific antibody directed against an epitope to the rat LPA3 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA3 receptor from mouse, rat, and human samples.

DETAILS

  • Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
  • Host: Rabbit
  • Clone: NA
  • Label: Unconjugated
  • Purity: Affinity purified on immobilized antigen.
  • Target: Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32
  • Comment: Contact Alomone Labs for technical support and product customization
  • Gene Id: 66025
  • Isotype: Rabbit IgG
  • Homology: Mouse - identical; human - 12/14 amino acid residues identical
  • Is Toxin: No
  • Sequence: (C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor
  • Synonyms: Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32
  • Accession: Q8K5E0
  • Clonality: Polyclonal
  • Lead Time: 1-2 Business Days
  • Reactivity: Human|Rat|Mouse
  • Formulation: PBS pH7.4
  • Applications: WB
  • Preservative: No Preservative
  • Reconstitution: 0.2 ml double distilled water (DDW).
  • Blocking Peptide: BLP-LR033
  • Negative Control: BLP-LR033
  • Positive Control: NA
  • Immunogen Location: Extracellular, N-terminus
  • Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
  • Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
  • Immunogen Source Species: Rat
  • Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
  • Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
  • Applications May Also Work In: WB
  • Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
  • Product Page - Scientific Background: Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptors have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2. All six receptors are type I, rhodopsin-like G-protein coupled receptors (GPCRs) that differ in their tissue distribution and downstream signaling pathways3.GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.Expression of LPA3 receptor has been observed in human heart, testis, prostate, pancreas, lung, ovary, and brain5 and is most abundant in mouse testis, kidney, lung, small intestine, heart, stomach, spleen, brain, and thymus6.LPA3 receptor null mice are viable and grossly normal, but female nulls show a striking phenotype in the reproductive system7. However, despite the fact that LPA3 is expressed in the frontal cortex, hippocampus, and amygdala, no phenotypes related to LPA3 loss in the nervous system have been reported to date5.
  • Standard Quality Control of Each Lot: Western blot analysis
  • Application Dilutions Western Blot Wb: 1:200
  • Antibody Concentration After Reconstitution: 1 mg/ml
  • Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information