DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: KMT1A, SUV39H1, Suppressor Of Variegation 3-9 Homolog 1 (Drosophila), Su(Var)3-9 Homolog 1
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Assay Kit
• References: Dillon S.C., et al. 2005. Genome Biology 6:227.
• Description: The SUV39H1 Chemiluminescent Assay Kit is designed to measure SUV39H1 activity for screening and profiling applications. The key to the SUV39H1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibodys followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
• Methodology: Chemiluminescent
• Supplied As: Kit comes in a convenient format, with 8-well strips precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, an
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 52008L, 53011, 51080, 51070
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Methyltransferase
• Instructions for Use: See assay kit datasheet for detailed protocol.