Polyclonal Antibody to Zap-70 (Ab-319)

DESCRIPTION:

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448 Ku GM, et al. (2001) EMBO J; 20(3): 457-465 Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780 Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774 Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844 Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.

DETAILS

Gene: ZAP70
App Id: WB,IHC
Antigen: Zap-70 (Ab-319)
Gene Id: 7535
Isotype: Rabbit IgG
Format Id: Purified
Uniprot Id: P43403
Host Animal: Rabbit
Clonality Id: Polyclonal
Reactivity Id: Human,Mouse,Rat
Product Content: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Alternative Name: 70 kDa zeta-associated protein|| SRK|| Syk-related tyrosine kinase|| ZA70|| ZAP-70
Application Note: Predicted MW: 70kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Categories Name 1: Antibodies
Categories Name 2: Cell Signalling
Storage Condition: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.