DESCRIPTION

• Notes: To generate a GPC3 stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of puromycin (as pre-determined from a killing curve) for antibiotics selection of transduced cells. Visit bpsbioscience.com/cell-line-faq for guidelines on performing a kill curve. Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
• Uniprot: P51654
• Shiptemp: -80°C (dry ice)
• Synonyms: Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, OCI5, Glypican-3 alpha subunit, Glypican-3 beta subunit
• Warnings: Avoid freeze/thaw cycles
• Category: Cell Signaling/Lentivirus
• Background: GPC3 (Glypican-3, also known as OCI5) is a cell surface proteoglycan highly expressed in the lungs, liver, and kidneys. Its function is tissue-dependent and can either promote or suppress tumorigenesis. Being a heparin sulfate proteoglycan, it is overexpressed in neoplasms including malignant melanoma, hepatocellular carcinoma, and testicular yolk sac tumors in which it regulates cell growth and differentiation. It is also involved in the negative regulation of the hedgehog signaling pathway and in the positive regulation of the non-canonical Wnt signaling pathway.
• Description: The GPC3 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human GPC3 (Glypican 3, NM_ 004484.2) driven by an EF1A promoter and a puromycin selection marker (Figure 1). Figure 1: Schematic of the lenti-vector used to generate the GPC3 Lentivirus.
• Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
• Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: Generate stable cell line expressing human GPC3 with puromycin selection
• Product Type: Lentivirus
• Biosafety Level: BSL-2
• Related Products: 78716, 78714, 78712, 78710
• Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. 
• Scientific Category: Cell Signaling Pathway