DESCRIPTION

• Aa: 530-end
• Mw: 38.5 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_005592
• Species: Human
• Uniprot: O15146 Q13255
• Shiptemp: -80°C (dry ice)
• Synonyms: MUSK, muscle, skeletal, receptor tyrosine kinase, Muscle-Specific Kinase Receptor
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Receptor Tyrosine
• References: 1. Zhou H. et al., J. Cell Biol. 146 (5), 1133-1146 (1999). 2. Evoli A. et al., Ann. N. Y. Acad. Sci. 1132, 76-83 (2008).
• Description: Human MUSK (GenBank Accession No. NM_005592), a.a. 530-end with N-terminal His-tag, MW = 38.5 kDa, expressed in Baculovirus infected Sf9 cell expression system.
• Formulation: 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Add 50 µl reaction mix (MUSK diluted in 1x kinase buffer supplemented with 0.2 mM ATP) to wells coated with substrate. Incubate for 1 hour. Add Europium-labeled anti p-Tyr antibody and incubate for 1 hour. Time resolved fluorescence measured at room temperature at λexc=330+/-40 nm and λem=620+/-10 nm.
• Related Products: 40084, 100927, 101115, 78286, 78487
• Specific Activity: 6.9 pmole/min/mg
• Storage Stability: >12 months at -80°C
• Scientific Category: Kinase/Serine-Threonine