DESCRIPTION

• Aa: full length
• Mw: 107 kDa (calculated)
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_006622
• Species: Human
• Uniprot: Q9NYY3
• Shiptemp: -80°C (dry ice)
• Synonyms: SNK, polo-like kinase 2
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• Background: PLK2 or Polo-like kinase 2 is a serum-inducible kinase that is a member of the Polo family of serine/threonine protein kinases which play an essential role in normal cell division. PLK2 phosphorylates the P4.1-associated protein (CPAP), a human homologue of SAS-4, in procentriole formation during the centrosome cycle. PLK2 may also play a role as a tumor suppressor since it interacts with TSC1 protein which in turn inhibits mTOR signaling, tumor growth and chemosensitivity under hypoxic conditions. Human lung tumor cells deficient in PLK2 grow larger than control tumors, and PLK2 interacts with endogenous TSC1 protein in these tumors.
• References: 1. Syed, N. et al., Blood. 2006 Jan 1,107(1):250-6. 2. Eckerdt, F. et al., Oncogene. 2005 Jan 10,24(2):267-76.
• Description: Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST-tag, expressed in a baculovirus infected Sf9 cell expression system. Theoretical MW is 107 kDa.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of substrate per minute at pH 7.5 and 30°C.
• Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200µM ATP, 0.01% Brij-35 and 2 µM substrate (SER/THR 16, Invitrogen) at room temperature for 1 hour.
• Related Products: 40035, 40033, 40236, 78487
• Specific Activity: 3.3 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine