NAO is a mitochondrial dye whose staining is not dependent on mitochondrial membrane potential, unlike JC-1 and Rhodamine 123. NAO has been used to study multidrug resistance and to measure changes in mitochondrial mass during apoptosis.    &lamba;Ex/&lamba;Em (MeOH) = 495/522nm  ε (MeOH) = 63,000  Orange solid soluble in DMSO or DMF  Store at 4°C and protect from light, especially in solution  C26H38BrN3  MW: 473  [75168-11-5]